关键词：
Plant sciences
Bioinformatics
Genetics
摘要：
Sweetpotato is an important staple food that feeds millions, but despite our understanding of its genetic architecture and the inheritance of important traits has lagged behind that of most other major crops. Recent advances in polyploid quantitative genetics and bioinformatics have facilitated entry into a new era where quantitative trait loci (QTL) and candidate genes for specific traits in polyploid crops such as sweetpotato are becoming more feasible. These new tools have become critical components for advancing cultivar development by helping us to understand the genetic factors underlying both simple and complex traits in sweetpotato. In this study, a biparental mapping population (DC) was created by crossing the light yellow-fleshed, high dry matter breeding line NCDM04-0001 (female) with the orange-fleshed, low dry matter cultivar Covington (male). Genotyping by sequencing (GBS), via a modified protocol called GBSpoly, was used to identify high-quality single nucleotide polymorphisms (SNPs) in the DC population from Illumina sequence data. The SNPs were analyzed utilizing novel genetic linkage mapping and QTL analysis algorithms (MAPpoly and QTLpoly respectively) for complex polyploids to develop a highdensity phased and integrated genetic linkage map of the DC population made up of 15 linkage groups that was phenotyped for important sweetpotato traits including the disease Fusarium oxysporum ***. batatas (Fob), and the storage root quality traits β-carotene, sucrose, glucose, and fructose. Analyses of the mapping population revealed two significant QTL for Fob on linkage groups 10 and 3, responsible for ~34% and ~10% of total phenotypic variation within the progeny. Based on the combined molecular and phenotypic data it is hypothesized that resistance to Fob is a polygenic trait in sweetpotato, a finding common in plant disease resistance studies. For β-carotene, sucrose, and fructose we identified three QTL each on linkage groups 2, 3, and 12: 1) On linka
万方期刊
CNKI期刊
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